FLIPR Tetra systems are fluorescence- and luminescence-based microplate readers with an integrated 1536-, 384- or 96-pipettor. Detection of KASP genotyping reactions For the second test, the validation kit contained 36 known samples, including 33 DNA samples and 3 no-template controls (NTC). Molecular Devices launches custom organoid line expansion service using unique bioprocess technology for high-throughput applications. Avoid losing high signal. 40266 Email: drugdiscoverytech@lifetech. Figure 10: The Plate section Template Editor, showing the series set up. After reading the ELISA plate on both readers, each standard curve was nearly identical. 1). Plus 384. (Molecular Devices cat. 00. Our specialized plate readers for absorbance, fluorescence, and luminescence detection provide optimal sensitivity. SoftMax Pro Software. Learn how you can utilize microplate readers to drive landmark discoveries. Table of Contents. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. These lab workhorses are used in drug discovery. Read plate standard and mix. It was then read from the bottom on a SpectraMax ® i3 Multi-Mode Microplate Reader using a preconfigured protocol in SoftMax Pro Software with the settings indicated in Table 1. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. Spectranet customer portal. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. Company Molecular Devices, LLC. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. 1 sec. 1. A plate reader’s software may offer automated analysis using protocols configured for a particular assay. $2,800. July 17, 2023. Molecular Devices SoftMax ® Pro software was used to analyze and present the data. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. 00. Molecular Devices is one of the leading provider of high-performance bioanalytical measurement solutions for life science research, pharmaceutical and biotherapeutic development. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Preventive Maintenance (PM) Each plate reader or washer is calibrated, inspected, and lubricated— any potential issues are. . : 8 Oct 12 Page 1 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. #ABS Plus) 4s3 ™ Semi-Automatic Sheet Heat Sealer (Brooks Life Sciences cat. Additional high-quality filters to supplement your assay wavelength range requirements are available directly from Molecular Devices. Filter 320/100 (available from Molecular Devices) Filter 616/10 (Molecular Devices cat. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. Use a Phillips screwdriver to remove the screws, and then remove the plate from the instrument. Company Info. Imaging System Giles Scientific Biomic V3 Automatic Plate. Plate pre-read measurements were made in a Costar UV plate. The VMax® Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm–750nm), with 4 filter choices included at time of purchase. First choose Read Mode ‘FL’ and Read Type ‘Endpoint’, and then select. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. Learn how Molecular Device's VersaMax Microplate Reader can be combined with the Pyrochrome Assay from Associates of Cape Cod, Inc. M5. Research-grade results. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. Are you looking for a versatile and affordable microplate reader that can perform various assays and measurements? Check out the FilterMax F3 and F5 Multi-Mode Microplate Readers, which offer absorbance, fluorescence, luminescence, and more read modes with high performance and reliability. It also integrates with the. Step 2: Launch SoftMax Pro Software. This multi plate reader is excellent to work with for checking the U. #0200-7009) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax i3x reader are shown below. Microplate Readers. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Microplate Reader, Multimode. Plate Reader. Select the Driver tab. 1. D SpectraMax ® M2 SpectraMax ® M2 e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. #iD3) Methods. ~ Bio-Tek ELx808 Microplate Reader $2,795. ELISA Plate Readers / Microplate Readers. , target CV per well = 1. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340–850nm). For more sample throughput, the system can be easily integrated into a full robotic to that of dedicated single-mode readers. The plate reader temperature was set to 37°C and allowed to reach 37°C before proceeding. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. Temperature control and shaking come standard. plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. 5. IMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers. The content of this site is published by the site. Endpoint, kinetic, spectrum. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. The guide also covers the software features, the specifications, and the accessories of the systems. EUROPE. This can increase throughput and efficiency, thus saving time and space in the lab. SpectraMax® Plus 384 Absorbance Plate Reader, Molecular Devices. One plate carrier adapter is provided with the instrument. Plate stacker and robot integration The Gemini EM Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. As new assays are developed or your needs change, simply add a detection cartridge like ScanLater™ Western Blot System or the SpectraMax® MiniMax™ Imaging Cytometer option as opposed to buying a brand new system. Perform the following steps to install the drivers: Start > Control Panel > System. It is more inert and reduces compound interference. The. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. . Combined with the analysis capabilities of SoftMax® Pro Software, we provide quantitation in an easy-to-read, user-customizableSpectraMax ABS Plus Microplate Reader (Molecular Devices cat. Supplier: Molecular Devices. (1) Repertoires of SARS-CoV-2 epitopes targeted by antibodies vary according to severity of COVID-19 Virulence December 31, 2022 David J. A standard spectrophotometer measures the absorbance of one sample at a time. 0) at 37 °C, fluorescence intensity was recorded on a Spectramax M5 Multimode plate reader (Molecular Devices, USA) at each timepoint. 1. Recombinant protein expression Nucleocapsid. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. The FlexStation uses a Xenon flashlamp and monochromators for both excitation and emission light to allow the use of a wide range of. #6590-0118) Filter 665/10 (Molecular Devices cat. 18 kg. Devices PathCheck® Sensor—the only patented technology available that measures the depth (optical pathlength) ofThe plate was covered and mixed on an orbital shaker for five minutes and then placed in a SpectraMax i3x reader and mixed. 9000-0756. Plate chamber design of the SpectraMax category 1 microplate readers. 1. Fluorescent Imaging Plate Reader (FLIPRTM ) technology to measure Ca2+ flux in cells was an important development in the early 1990’s and has played a significant role in. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. Supplier: Molecular Devices. Molecular Devices SpectraMax Gemini XS Microplate Fluorometer. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed. Molecular Devices’ unique SpectraDrop™ Micro-Volume Plate offers a high throughput and accurate solution for low volume measurement. Molecular Devices SpectraMax® microplate readers support a multitude of assays for nucleic acids and protein detection. FlexStation™ II Systems provide integrated fluid transfer capability from a source plate or reservoir to an assay plate with concurrent kinetic fluorometric analysis of reactions. 315 can't find zero order. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. #0200-7009) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax i3x reader are shown below. Uncontrolled Copy for Training and Reference Purposes Only . The FlexStation 3 reader exclusively offers fluid transfer validation using Molecular Devices patented PathCheck® Feature to quantify the integrated 8- and 16-channel pipettor head performance. Supersedes 1. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. These applications may make use of…FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one. 50-plate magazine : 0200-6187. Shop now. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. ELISA Workflow Using the EMax plus Microplate Reader and MultiWash+ Microplate Washer. 4 °C above ambient to 45 °C. Cells in each. Also refer to the Multimode Reader Comparison Chart Features. Supersedes 1. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. A. The ultimate guide to microplate readers is an eBook that tells you everything you need to know. Fixed cells stained with fluoresceinconjugated anti-VCAM are identified with the Marker Expression Protocol. On the back, in the right hand top corner, there. The plate reader can measure multiple readouts, including UV and. Customize your instruments, as well as automate entire workflows to meet the specific needs of your assay, method, or. Plate Formats: 6 to 384-well microplates. Reads standard cuvettes & 96-384 well plates from 190 to 1000 nm. Laboratory automation workflows can help. Incubate at RT. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. 세포신호전달은 세포가 환경에 반응하고 다른 세포와 소통할 수 있게 합니다…. 0077 OD 0. The instrument supports the following read modes:. The purposes of the study were: 1) to determine the optimal wavelength settings for three cell lines. StakMax Handling Stacker Base Unit. 76358-624. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Superior optics Microplate Reader Shelf. A control well was set up to verify the accuracy of the standard curve. Learn how you can utilize microplate readers to drive landmark discoveries. AXON Patch-Clamp. 005 EU/mL to 5. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. 8×15×8. Contact our support team with your questions regarding Molecular Devices products and applications. #6590-0137) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax iD5 reader are shown below. To update the driver. The adapter is required for optimum performa nce with standard 96-well and 384-. The following components are located on the back panel of the SpectraMax and VersaMax readers: • Power switch:Chapter 1: Introduction The SpectraMax iD3 Multi-Mode Microplate Reader from Molecular Devices is a monochromator-based, multi-mode plate reader. Molecular Devices, LLC. Integrated stacker option. Lower variance: Normalize your plate reader assay data to the number of cells in each well. Read More. STAKMAX. Upgradeable modules are also available. 0. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. The temperature in the plate chamber is ambient and isothermal. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. In addition, users can define individual reagents and concentrations to be delivered to each well. PathCheck sensor corrects optical pathlength & pipetting variation. 2. Absorbance Plate Reader Comparison Chart. washer including mechanical, electrical, and optical components to verify operating functions are in accordance to the Molecular Devices product operational specifications. The SpectraMax iD3 User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax iD3 Multi-Mode Microplate Reader, a versatile instrument that measures absorbance, fluorescence, and luminescence. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. It will operate in 96 and 384 well plate format, and has installed filters for fluorescence of 370, 485 and 535 nm for excitation. We would like to show you a description here but the site won’t allow us. Samples with volumes as low as 2 µL can be measured. The plate was incubated at room temperature for 50 minutes or 2. Select the Driver tab and click Update Driver. 00 USD. Our microplate readers are the industry’s most cited instruments and have empowered life science researchers to advance protein and cell biology, breaking the barriers to novel, landmark discoveries. FlexStation™ II Systems provide integrated fluid transfer capability from a source plate or reservoir to an assay plate with concurrent kinetic fluorometric analysis of reactions. When connecting for the first time to either the SpectraMax L reader or StakMax system. Used. Since cells are nearly confluent, the protocol. 0 . SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Fill a clean plate with water. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. Shop a wide selection of Molecular Devices FLIPR TETRA System's on LabX. (b) Fluorescence responses of probe HADP and control compounds 2–4 (each 5 µM) towards human heparanase (HPA) (1 µg) in 40 mM NaOAc buffer (pH 5. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. The VersaMax Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. Preventive Maintenance (PM) Each plate reader or washer is calibrated, inspected, and lubricated—any potential issuesPlate reader instructions Computer. book Page 1 Tuesday, July 6, 2010 2:08 PM Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. 0%, raw data units = counts/sec. Plate reader. A preconfigured. Retrieving Each. System setup and calibration are controlled from within SoftMax Pro Software. or 0. Devices’ StakMax® Microplate Stacker in a matter of minutes and . The plate is set up for reading and images are acquired according to specified parameters. Cellular Imaging Systems. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. Every step is optimized to simplify analysis and reporting to support our microplate readers. Molecular Devices has demonstrated the value of a variety of ELISAs for a wide range of research approaches,. After reading the ELISA plate on both readers, each standard curve was nearly identical. Page 1 of 8 . In . Instrument settings can be saved as a protocol file. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. 1 - 16 of 69. Select Start>Control panel>Device Manager. Shortside barcode reader: 0310-5102. Step 1: Capture antibody binds to ELISA plate wells. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Never order or replace filters to optimize an assay again!SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. Pre-defined protocols. Three low volume 24-well bottom slides. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Microplate Reader Shelf. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). 5. The EMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm), with 4 filter choices included at time of purchase. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. BioTek Synergy H1 Hybrid OMRF research scientist Kumar Subramani reviewed BioTek Synergy H1, stating it is a configurable multi-mode microplate reader. Also refer to the Multimode Reader Comparison Chart Features. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. Narrow bandwidths deliver increased accuracy and enhanced peak resolution. 2. Supplier: Molecular Devices. Turn on Plate Reader and wait for it to complete its start-up routine. A single software solution addresses your needs, from plate set-up to image analysis. Cells in each image are identified by SoftMax Pro Software and cell-by-cellOne plate carrier adapter is provided with the instrument. Introduction. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. The absorbance (A405) can be measured by the plate reader and is proportional to the endotoxin concentration in the sample. 001 EU/mL) was added in order to test the sensitivity of the SpectraMax i3 reader. Description Figure 1. integration time per well, depending on firmware version. 1. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. 1 second up to 30 seconds. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. To update the driver. SoftMax® Pro 7. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. Select the Driver tab. FAST AND ACCURATE METERTECH Microplate Reader $3,650. An industry-renowned instrument for monitoring GPCRs and ion channels. Videos & Webinars. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes wi. Increase productivity with patented microplate reader technologies. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, and Fig 2: Enzymatic response of synthetic compounds. AXON Patch-Clamp. 190. SpectraMax Absorbance, Fluorescence. Lamp source is a Xenon flash lamp (1 Joule/flash). Our luminescence plate readers, also referred to as luminometers are ideal for measuring a wide range of assays including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence resonance energy transfer. 9. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. SpectraMax® Paradigm Plate Reader Oksana Sirenko, Cathy Olsen, Vanessa Ott and Evan F Cromwell Molecular Devices, LLC, 1311 Orleans Drive, Sunnyvale, CA 94089 There has been a trend in the past decade from biochemical assays toward cell based assays. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. Chapter 1: Introduction The SpectraMax® iD3 Multi-Mode Microplate Reader from Molecular Devices is a monochromator-based, multi-mode plate reader. Clone Screening. 72 MB. Robust, high value microplate reader designed to deliver research-grade results to any laboratory. Turn on Plate Reader and wait for it to complete its start-up routine. 400×320×350 mm. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. Superior optical system includes a xenon flash lamp and features. Molecular Devices SpectraMax 384 Plus Microplate Reader. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Important things to consider when purchasing a multimode microplate reader. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. Mode Reader with the StakMax Microplate Handling System from Molecular Devices. 0A-Sunnyvale, CA-Made in USAMolecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. Sunnyvale, Calif. “Sensitivity is pretty much clutch for luminescence,” says Daly. W×D×H. The SpectraMax 190 reader can be integrated with Molecular . We would like to show you a description here but the site won’t allow us. Read height should be optimized for Vmax® plate reader specifications. S. Sunnyvale, CA - May 17, 2011 - Today, Molecular Devices, a leading provider of instruments, software, and consumables for drug discovery and life sciences research, announced that NanoRacks, LLC has chosen the SpectraMax® M5e Multi-Mode Microplate Reader for use in NASA's International Space Station; the M5e Reader will be the first. Page 1 of 8 . Used. Check software compatibility with Windows 10 and locate your software installer. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol ‘NanoOrange’. 2. Every step is optimized to simplify analysis and reporting to support our microplate readers. Molecular Devices' microplate readers. 2. This document is copyright protected and any reproduction of this document, in whole or. Accurate and sensitive detection of nucleic acids and proteins are critical to many experiments. 305 reference level saturation. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. It may be named "Pothos". Read one sample or up to 384 in a. 0A Sunnyvale, CA Made in USA Microplate readers read biological, chemical or physical events that occur on a microtiter plate. The computer controlling the plate reader runs Windows XP. 5577; General Inquiry Form; Mon-Fri 8:00am - 5:00pmFor the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. #0200-7011) Instrument setup SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. Tests every subsystem of the reader or . Step 4: Add detection antibody – Enzyme-conjugated detection antibody binds to a second site on the. • 0. #SpectraMax L Config). Reader Documentation Version No. Integrated to. 5 sec. Here, the CVs for each plate were less than 3, and the difference between mean onset time for the plates run on the two readers was only 7%. Timothy Bolus, MPM, MBA, Compliance Program Manager. The SpectraMax M2 and M2e are versatile and reliable instruments that can perform a range of applications in. The system provides automation for up to 50 microplates for easy walkaway automation. sensitivity or performance. , attenuation mode = out. Fluorescence Microplate Reader Comparison Chart. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. If it looks fine, then proceed. Experimental details and customization of the group table. Tristar 3 Multimode Microplate Reader. 5: Schematic of the back panel of a reader. Next generation SpectraMax® absorbance microplate readers. Gemini EM is a microplate spectrofluorometer from Molecular Devices. It features a modular design that allows users to add or change detection cartridges according to their needs. ELISA (Enzyme-linked Immunosorbent Assay) is one of the most popular quantitative methods to detect a target antigen such as toxin or foreign substance within a sample. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide 0112-0102 Rev. the standard plate reader applications, protecting your initial investment. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. coli stock with an OD 600 value of 0. Uncontrolled Copy for Training and Reference Purposes Only . SpectraTest® Validation Package User Manual Download Page. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more ( Molecular Devices ) equipment, software, reagents, and consumables to use in the operation of such Molecular Devices equipment, software, reagents, and consumables. Microplate Readers. Additional high-quality filters to supplement your assay wavelength range requirements are available directly from Molecular Devices. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. The sample is typically placed in a cuvette through which light is sent horizontally. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. 25 to 3. SoftMax® Pro 7. #4TI-0214) UltraVision Plate 96 (Brooks Life. Description 1. SpectraMax® 190 Absorbance Plate Reader, Molecular Devices. +1 800-635-5577. The ability to accurately quantitate cell number in multi-well microplates enables a multitude of biological applications that study cell health or proliferation. Alpha Technology capability can be added to the system at any time as a user’s detection needs evolve. With optimized reagents, option to operate the. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). D SpectraMax ® M2 SpectraMax ® M2 e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. (a) Chemical structures of 1 (HADP) and its analogues 2–4. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. Item SpectraMax i3x Multi-Mode Microplate Reader. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. The plate reader optics are tunable for absorbance,. Reader Documentation Version No. com 4. 100 μL of blank or endotoxin standard (in. These robust readers have been placed in labs from. The user guide also covers the features, specifications, and software of the reader, as well as the installation and. Instruments are configurable for the assay-specific detection modes and performance your laboratory needs. Plate Formats 6 to 1536 wells. With three modes of detection for full spectrum absorbance, fluorescence and luminescence, the SpectraMax® Mini reader . The plate is set up for reading and images are acquired according to specified parameters. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). For nearly 30 years, Molecular Devices has provided scientists with . US HEADQUARTERS. Data analysis. Ratios for each sample were calculated as Read B/Read A. The two different data import options are tailored to quickly get your data into SoftMax Pro to provide a single source of software for all your data reduction needs. Cells were thawed and propagated according to the recommended method specified by the supplier. Increase productivity with patented microplate reader technologies.